### PO 2024 ###
### Studiengang und Semester
5BBT, 6BBTPV
### Modulbezeichnung
Practical Molecular Biology
### Englische Modulbezeichnung
Praktikum Molekulare Biologie
### Art
Pflichtfach
### Modulsprache
English
### ECTS-Punkte
6
### Studentische Arbeitsbelastung
60, 120
### Voraussetzungen (laut Prüfungsordnung)
Molekulare Biologie / Molecular Biology
### Empfohlene Voraussetzungen
Praktikum Biochemie / Chemie der Biomoleküle
### Pruefungsform und -dauer
experimental work, protocolls, final kolloquium / experimentelle Arbeit (Studienleistung)
### Lehrmethoden und Lernmethoden
Practical / Praktikum
### Modulverantwortlicher
J.J. Reimer
### Qualifikationsziele
Students, that haven't finished the module 'Molekulare Biologie / Molecular Biology', may have the chance to join the practical after passing an entrance test.

At the end of the semester, the students will be able ...
- to generate genetically modified organisms (GMO),
- to conduct a cloning strategy,
- to transform (biological) cells with the help of different methods,

due to the reason that they ...
- used and applied the knowledge of restriction enzymes,
- analysed DNA-sequences,
- evaluate, analyse and discuss their results,

to later use this for ....
- improving processes to generate GMOs,
- and to develop analytical methods for GMOs.
### Lehrinhalte
Performing a restriction assay and a ligation assay, transformation of different bacteria via heat shock and electroporation, gel electrophoresis analysis, cloning phage DNA into a bacteria, PCR detection

The preferred language during the practical is english.
### Literatur
s scripts.
### Titel der Lehrveranstaltung
Practical Molecular Biology
### Dozent
J.J. Reimer
### SWS
4